Gemfibrozil is used to lower cholesterol and triglyceride levels in the blood and Ibuprofen is a common over-the-counter painkiller. Fast separations of these two compounds are obtained on short 50 mm columns. Both Primesep B2 and Primesep D separate by a combination of anion exchange and reversed phase. Retention times on Primesep B2 can be adjusted by changing the percentage of acetonitrile. On Primesep D retention times can be changed by adjusting the percentage of acetonitrile and acetic acid in the mobile phase. The HPLC separations use a mobile phase of water, acetonitrile (MeCN, ACN), ammonium formate and acetic acid (HOAc) with UV detection at 270 nm.
Application Column
Primesep B2
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsIbuprofen
