HPLC Analysis of Fatty Acids with MS Compatible Conditions


Fatty acids are carboxylic acids with a long unbranched carbon chain. They are hydrophobic compounds with different number of carbons. Most naturally occurring fatty acids have a chain of an even number of carbon atoms. Three fatty acids are separated on a Primesep 100 HPLC column by reversed-phase mechanisms. This general HPLC method and mixed-mode column can be used for separation of other fatty acids.

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Myristic Acid
Palmitic Acid
Stearic Acid
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.