HPLC Analysis of Drugs in Saliva

The presence of three drugs, dextromethorphan, amitriptyline and trimipramine in saliva were analyzed by mixed-mode chromatography on a Primesep D reversed-phase anion-exchange column. No sample preparation is required as proteins from saliva are not retained at these conditions and elute in the void of the column due to cation-exclusion effect. Both column and proteins are positively charged at current conditions. Mobile phase consists of ACN-water-formic acid and is compatible with LC/MS detection technique. This general HPLC method can be used for analysis of hydrophobic basic drugs in biofluids.

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:


Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.