HILIC Separation of Surfactant Triton X-100 on Obelisc N Column

Triton X-100 is a non-ionic surfactant which has a hydrophilic polyethylene oxide group (on average it has 9.5 ethylene oxide units), and a hydrocarbon lipophilic or hydrophobic group. Obelisc N HPLC columns are used to separate oligomers of Triton X-100. Separation is achieved by hydrophilic interaction chromatography. This method can be used in determination of oligomers of Triton X-100 in detergents, liquid, paste, and powdered cleaning formulations. This separation can be monitored with almost any detection technique (ultra-violet, ELSD, LC/MS, RI, CAD, etc.)

Condition

Column Obelisc N,  4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 5.0
Flow Rate 1.0 ml/min
Detection UV 250, 230nm

 

Description

Class of Compounds
Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Triton X-100

Application Column

Obelisc N

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Triton X100

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.