Separation type: Liquid Chromatography HILIC
Ritonavir and Ribavarin are two drugs which are very different in their properties for HPLC separation. Very hydrophobic and very hydrophilic drugs are separated on Primesep N using Hydrophilic Interaction Chromatography (HILIC). Ritonavir is an antiretroviral drug from the protease inhibitor class used to treat HIV infection and AIDS. Ritonavir is very hydrophobic and non-ionizable and retains very long in reverse phase chromatography. Ribavirin is an anti-viral drug which is active against a number of DNA and RNA viruses. It is a member of the nucleoside antimetabolite drug, which makes it very hydrophilic.
Column | Primesep N, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 97/3% |
Buffer | No |
Flow Rate | 1.0 ml/min |
Detection | UV 250 nm, MS- compatible mobile phase |
Class of Compounds | Drug |
Analyzing Compounds | Ritonavir, Ribavirin |
Application Column
Primesep N
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsRitonavir