Most reversed-phase HPLC columns, such as C18, are incompatible with 100% aqueous mobile phases due to dewetting (collapse) of the stationary phase. Primesep columns combine reversed-phase with polar functional groups which eliminate the problems of dewetting. The Primesep 100 column in this application with an acetonitrile (MeCN, ACN) shows a retention time of 2.5 minutes. In 100% water, the retention time is 3 minutes even after sitting 24 hours in the water mobile phase. On a C18 phase, the analyte retention would have been lost and elution at the void (1.5 minutes) would have resulted.
| Column | Primesep 100 |
| Mobile Phase | MeCN/H2O |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
Primesep C
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
