Benfluralin Analysis on Obelisc R HPLC Column

Benfluralin is a dinitroaniline herbicide used on grasses and weeds. The EURL (European Union Reference Laboratory) included benfluralin in a study using a multiresidue method by GC-MS/MS in olive oil. Obelisc R was used to separate benfluralin and its impurities using a single multiresidue method, which can be used for many pesticides. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning. Method is LC/MS compatible and can be used as a general approach for analyzing benfluralin and dozens of other pesticides.


Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4.5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm




Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Benfluralin


Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.