Primesep 100 retains an amino sugar by a combination of cation exchange and reversed phase. Columns with reversed-phase functionality alone are unable to retain this amino sugar. The polar interactions of the Primesep 100 column together with the reversed-phase interactions retain the amino sugar and resolve it from its many impurities. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and sulfuric acid (H2SO4) with UV detection at 205 nm.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | H2SO4 |
| Flow Rate | 1.5 ml/min |
| Detection | UV 205 nm |
| Class of Compounds |
Hydrophilic, Ionizable |
| Analyzing Compounds | Sugars |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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