HPLC Analysis of Dichloran

Dichloran (DCNA) is a fungicide with low toxicity in humans, but is highly toxic to freshwater fish. Obelisc R was used to retain DCNA and separate it from impurities. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing DCNA.


Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 40%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection UV, 350 nm


Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Dichloran
Application Analytes: