HPLC Analysis of Azinphos-ethyl

Azinphos-ethyl is an organophosphate insecticide similar to Azinphos-methyl which was developed during World War II as a nerve agent. Due to risk concerns for farm workers and aquatic ecosystems AZM use was phased-out by the EPA in 2012. AZM is on the EURL–SRM list of target pesticides in cereals and feeding stuffs. Azinphos-ethyl was retained on Obelisc R, a mixed-mode HPLC column which contains ionic groups and hydrophobic chain. Method is LC/MS compatible and can be used as a general approach for analyzing azinphos-ethyl and other organophosphate insecticides.


Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 40%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Azinphos-ethyl
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Application Analytes:

Application Detection:
UV Detection