Histamine

Histamine structural formula

CAS Number51-45-6
Molecular FormulaC5H9N3
Molecular Weight111.149
InChI KeyNTYJJOPFIAHURM-UHFFFAOYSA-N
LogP-0.700
Synonyms
  • Histamine
  • 2-(1H-Imidazol-5-yl)ethan-1-amine
  • 1H-Imidazole-5-ethanamine
  • 51-45-6
  • 1H-Imidazole-4-ethanamine
  • 1H-Imidazole-5-ethanamine
  • 2-(1H-Imidazol-4-yl)ethanamine
  • 2-(1H-Imidazol-4-yl)ethylamine
  • 2-(1H-Imidazol-5-yl)ethanamine
  • 2-(1H-Imidazol-5-yl)ethylamine
  • 2-(3H-Imidazol-4-yl)ethylamine
  • 2-(4-Imidazolyl)ethanamine
  • 2-(4-Imidazolyl)ethylamine
  • 2-imidazol-4-iletilamina
  • 2-Imidazol-4-ylethylamin
  • 2-Imidazol-4-ylethylamine
  • 2-imidazole-4-ylethylamine
  • 4-(2-Aminoethyl)-1H-imidazole
  • 4-(2-Aminoethyl)imidazole
  • 5-Imidazoleethylamine
  • Ergamine
  • Ergotidine
  • Imidazole-4-ethylamine
  • NSC 33792
  • β-Imidazolyl-4-ethylamine
  • EINECS 200-100-6
  • Ethylamine, 2-imidazol-4-yl-
  • Imidazole, 4-(2-aminoethyl)-
  • Istamina
  • UNII-820484N8I3
  • 2-(1H-imidazol-4-yl)ethan-1-amine
  • 2-Imidazol-4-yl-Ethylamine
  • 4-Imidazoleethylamine
  • Eramin
  • Free histamine
  • HSM
  • L-Histamin base
  • L-Histamine
  • Theramine
  • b-Imidazolyl-4-ethylamine
  • beta-Aminoethylglyoxaline
  • beta-Aminoethylimidazole
  • beta-Aminothethylglyoxaline
  • beta-Imidazolyl-4-ethylamine
  • 64422-25-9
  • 924364-91-0

Applications:

HPLC Method for Determination of Histamine on BIST A+ Column by SIELC Technologies

October 18, 2022

HPLC Method for Determination of Histamine on BIST A+ Column by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Determination of Histamine on BIST A+ Column
HPLC Method for Determination of Histamine on BIST A+ Column by SIELC Technolog

High Performance Liquid Chromatography (HPLC) Method for Analyses is of Histamine BIST A+ Column by SIELC Technologies

Histamine dihydrochloride, also known as Ceplene, is used to prevent a relapse of acute myeloid leukemia. Other pharmaceutical applications include as a topical analgesic to treat arthritis, aches, bruises, sprains, and strains. Brand names it is sold under include Australian Dream and Alo. Histamine dihydrochloride can be detected in the low UV regime. Using a BIST A+ column and a mobile phase consisting of water and Acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, Histamine chloride can be retained, separated, and analyzed. This analysis method can be detected and is compatible with Mass Spectrometry (MS), ELSD, and CAD. 

Condition

ColumnBIST A+, 4.6×150 mm, 5µm, 100A
Mobile PhaseMeCN – 70%
BufferAmmonium Formate pH 3.0 – 20 mM
Flow Rate1.0 ml/min
DetectionELSD, 50C

Description

Class of CompoundsDrug
Analyzing CompoundsHistamine

Application Column

BIST A+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
Histamine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Histamine and Phenethylamine on Primesep C Column

August 7, 2010


Histamine is a basic compound that acts like neurotransmitter. Phenethylamine is a natural amine alkaloid psychoactive compound with stimulant effects. It forms by enzymatic decarboxylation of phenylalanine, and also acts as neurotransmitter. Both compounds are separated in one HPLC run on Primesep C and Primesep 200 columns by reversed-phase cation-exchange mechanism. Primesep C provides shorter retention time and histamine and phenethylamine can be separated within 5 minutes. Primesep C and Primesep 200 columns do not require ion-pairing reagent as it is attached to the surface of Primesep silica gel. Method can be used for analysis of these two compounds in plasma, blood and urine with UV, ELSD, CAD or LC/MS detection.

Application Column

Primesep C

Column Diameter: 3.2 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Histamine
Phenethylamine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Histamine and Phenethylamine on Primesep 200 Column

June 12, 2004


Histamine is a basic compound that acts like neurotransmitter. Phenethylamine is a natural amine alkaloid psychoactive compound with stimulant effects. It forms by enzymatic decarboxylation of phenylalanine, and also acts as neurotransmitter. Both compounds are separated in one HPLC run on Primesep C and Primesep 200 columns by reversed-phase cation-exchange mechanism. Primesep C provides shorter retention time and histamine and phenethylamine can be separated within 5 minutes. Primesep C and Primesep 200 columns do not require ion-pairing reagent as it is attached to the surface of Primesep silica gel. Method can be used for analysis of these two compounds in plasma, blood and urine with UV, ELSD, CAD or LC/MS detection.

Application Column

Primesep 200

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Histamine
Phenethylamine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.