Bromadiolone

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Bromadiolone a rodenticide meant to kill rats and mice. It’s sold as an odorless powder with a light yellow tint of color. It is toxic if ingested orally since it the blood clotting due to the rodenticide not letting the body to reuse the K Vitamin. Primesep 100, a reverse-phase column, contains embedded acidic ionizable groups and can retain Bromadiolone. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.