Ribulose 1,5 Bisphosphate

CAS Number14689-84-0
Molecular FormulaC5H12O11P2
Molecular Weight310.088
InChI KeyAAAFZMYJJHWUPN-SOOFDHNKSA-N
LogP-4.7
Synonyms
  • Ribose-1,5-diphosphate
  • ribose-1,5-bisphosphate
  • D-Ribofuranose, 1,5-bis(dihydrogen phosphate)
  • D-Ribose 1,5-bisphosphate
  • 1,5-di-O-phosphono-D-ribofuranose
  • D-ribofuranose 1,5-bis(dihydrogen phosphate)
  • 14689-84-0
  • AC1MI1JN
  • SCHEMBL1332683
  • CHEBI:17994
  • D-RIBULOSE 1,5-DIPHOSPHONATE

Applications:

HPLC Separation of Sugar Phosphates

October 14, 2010


Sugar phosphates occur in biological systems. Sugar phosphates are parts of two metabolic pathways: glycolisis and pentose phosphate pathways. One of the purposes of sugar phosphates is to store and transfer energy in cells. Because of the presence of sugar fragment and phosphate moiety these molecules are very polar in nature. Mixed-mode chromatography was applied for separation of glucose and fructose phosphates and diphosphates. Compounds are separated based on anion-exchange properties with diphosphates retaining longer than mono phosphates. Monitoring is done by ELSD.

 

 

Condition
Column Primesep SB, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer AmFm – pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD

 

Description
Class of Compounds
 Sugars
Analyzing Compounds Sodium, Glucose 6- phosphate, Fructose 6 –phosphate, Fructose 1.6 biphosphate, Ribulose 1.5 biphosphate

 

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Fructose 1,6 Bisphosphate
Fructose 6-Phosphate
Glucose 6-Phosphate
Ribulose 1,5 Bisphosphate
Sodium
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.