The Legacy L8 column is a single-mode, ion-exchange column with aminopropylsilane (AP) groups chemically bonded to totally porous silica gel micro-particles. The Legacy L8 column meets the UPS standard for the L8 designation. The AP groups allow for both cation-exclusion and hydrophilic interaction chromatography (HILIC).
Legacy L8 columns are available in all standard dimensions.
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um
Pores: 100 A
If you would like to learn more about SIELC’s line of Legacy columns, you can download the brochure here.
Short-chain peptides can often be highly polar, and when they include lysine moieties, they can be very basic as well. Short-chain lysine-based peptide oligomers have been found to have their best peak shape in HILIC/cation-exclusion mode. Figure 40 shows how these oligomers of different sizes (one made from 3 lysine moieties and one made from 6) can be retained on a Legacy L8 column using a gradient mobile phase consisting of MeCN, water, and phosphoric acid as a buffer.
Polylysine, significantly longer-chain polymers made out of many more lysine units, are easily produced by batcerial fermentation. Since any given sample can have differently sized polymers, it is often difficult to obtain a single, sharp peak. Figure 41 shows, however, that a relatively sharp peak can be obtained on a Legacy L8 column with a gradient mobile phase consisting of MeCN, water, and phosphoric acid as a buffer.
Legacy L8 column meets the USP definition with a stationary phase that consists of octadecyl silane chemically bonded to porous silica or ceramic particles (1.5 to 10µm).
Legacy L8 columns are available in all standard dimensions.
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um
Pores: 100 A
If you would like to learn more about SIELC’s line of Legacy columns, you can download the brochure here.
