The Legacy L3 column is a single-mode, normal phase column that contains porous silica, without any ligands or additional chemistry attached to the surface. The Legacy L3 column meets the USP qualifications for the L3 designation. The chromatograms in this section show injections done according to USP standard methods for the given compounds, with modifications to the methods noted where applicable.
Legacy L3 columns are available in all standard dimensions.
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um
Pores: 100 A
If you would like to learn more about SIELC’s line of Legacy columns, you can download the brochure here.
Sulfuric acid is a highly corrosive acid commonly used as a buffer in chromatography. Sulfamic acid is an intermediary between sulfuric acid and sulfamide. Figure 1 shows the separation and retention of sulfuric and sulfamic acid on a Legacy L3 column with a mobile phase consisting of MeCN, water, and formic acid. This separation also used a gradient to change the concentration of MeCN and formic acid over time.
Lecithin s are a class of amphiphilic, fatty, yellow-brownish compounds that occur naturally in plant and animal tissues. Soy lecithin is lecithin derived from soy, and is often used as an emulsifier in food production. Figure 31 shows how commercial-grade lecithin and soy lecithin can be retained on a Legacy L3 column with a mobile phase consisting of MeCN, water, and an ammonium formate buffer using a gradient to control the buffer and MeCN concentrations over the course of the separation.
Legacy L3 column meets the USP definition with a stationary phase that consists of octadecyl silane chemically bonded to porous silica or ceramic particles (1.5 to 10µm).
Legacy L3 columns are available in all standard dimensions.
Column IDs: 22 mm, 10 mm, 4.6 mm, 3.2 mm, 2.1 mm, 1 mm, 0.5 mm, 0.25 mm
Column Lengths: 250 mm, 150 mm, 100 mm, 50 mm, 25 mm, 10 mm
Particles: 5 um
Pores: 100 A
If you would like to learn more about SIELC’s line of Legacy columns, you can download the brochure here.
