HPLC Separation of Sorbic, Fumaric, Malonic Acids on Newcrom BH Column

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Acids with different strengths can be retained and elution order controlled by the use of a mixed-mode column, which has an ion-exchange component in addition to hydrophobic retention.  In this example, the Newcrom BH mixed-mode column was used to separate fumaric, malonic and sorbic acids in HPLC.  Sorbic acid was mostly retained by hydrophobicity, while fumaric and malonic acids were also retained by the adjustment strength of the mobile phase


Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection UV 200nm


Class of Compounds Acid
Analyzing Compounds Sorbic Acid, Levulinic Acid

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Fumaric Acid
Malonic Acid
Sorbic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.