HPLC Separation of Levulinic acid and Sorbic Acid on Newcrom BH Column

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Levulinic acid is used as a precursor for a wide variety of compounds and is used extensively in pharmaceuticals and cosmetics industries. Sorbic acid is used as a preservative in food and drinks. The two acids can be separated in HPLC using Newcrom BH mixed-mode column using a mobile phase of acetonitrile (ACN) and water and either phosphoric acid (H3PO4) or formic acid (FA) as buffer allowing the the use of either UV or MS detector.



Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H3PO4, Formic Acid
Flow Rate 1.0 ml/min
Detection UV 210, 266 nm


Class of Compounds Acid
Analyzing Compounds Sorbic Acid , Levulinic Acid

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Levulinic acid
Sorbic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.