HPLC Separation of Levulinic acid and Sorbic Acid on Newcrom BH Column

HPLC.cloud View on hplc.cloud HPLC.cloud View on hplc.cloud Levulinic acid is used as a precursor for a wide variety of compounds and is used extensively in pharmaceuticals and cosmetics industries. Sorbic acid is used as a preservative in food and drinks. The two acids can be separated in HPLC using Newcrom BH mixed-mode column using a mobile phase of acetonitrile (ACN) and water and either phosphoric acid (H3PO4) or formic acid (FA) as buffer allowing the the use of either UV or MS detector.  


Column Newcrom BH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H3PO4, Formic Acid
Flow Rate 1.0 ml/min
Detection UV 210, 266 nm


Class of Compounds Acid
Analyzing Compounds Sorbic Acid , Levulinic Acid

Application Analytes:
Levulinic acid
Sorbic acid