Levulinic acid is used as a precursor for a wide variety of compounds and is used extensively in pharmaceuticals and cosmetics industries. Sorbic acid is used as a preservative in food and drinks. The two acids can be separated in HPLC using Newcrom BH mixed-mode column using a mobile phase of acetonitrile (ACN) and water and either phosphoric acid (H3PO4) or formic acid (FA) as buffer allowing the the use of either UV or MS detector.
| Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | H3PO4, Formic Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210, 266 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Sorbic Acid , Levulinic Acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsApplication Analytes:
Levulinic acidSorbic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
