Dexamethasone is a corticosteroid that is used as a replacement of the natural hormone produced by adrenal glands when the body can’t produce enough on its own. It has also been found to reduce deaths among the critically ill patients with the COVID-19. Prednisolone is another corticosteroid used to treat a variety of inflammatory conditions and has a similar structure to dexamethasone. Both steroids can be retained and separated in HPLC using Newcrom A mixed-mode column with the mobile phase consisting of either acetonitrile (ACN) or methanol (MeOH) and water with formic acid buffer. Methanol offers longer retention time compared to acetonitrile. UV detection at 275nm.
| Column | Newcrom A , 3.2×100 mm, 3 µm, 100A |
| Mobile Phase | MeCN, MeOH |
| Buffer | Formic Acid – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 275 nm |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Dexamethasone, Prednisolone |
Application Column
Newcrom A
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsPrednisolone
