HPLC Method of Analysis for Dexamethasone and Prednisolone

View on hplc.cloud

Dexamethasone is a corticosteroid that is used as a replacement of the natural hormone produced by adrenal glands when the body can’t produce enough on its own.  It has also been found to reduce deaths among the critically ill patients with the COVID-19.  Prednisolone is another corticosteroid used to treat a variety of inflammatory conditions and has a similar structure to dexamethasone.  Both steroids can be retained and separated in HPLC using Newcrom A mixed-mode column with the mobile phase consisting of either acetonitrile (ACN) or methanol (MeOH) and water with formic acid buffer.  Methanol offers longer retention time compared to acetonitrile.  UV detection at 275nm.


Column Newcrom A , 3.2×100 mm, 3 µm, 100A
Mobile Phase MeCN, MeOH
Buffer Formic Acid  – 0.2%
Flow Rate 0.5 ml/min
Detection UV, 275 nm



Class of Compounds
Drug, Acid, Hydrophilic, Ionizable
Analyzing Compounds Dexamethasone, Prednisolone


Application Column

Newcrom A

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.