HPLC Method for Analysis of Tyramine on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Tyramine on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Separation of Tyramine on Primesep 100 Column
HPLC Method for Analysis of Tyramine on Primesep 100 Column by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Tyramine

Tyramine is a naturally occurring monoamine compound that belongs to the class of biogenic amines. It is derived from the amino acid tyrosine and is found in various foods. Tyramine is particularly known for its potential role in certain food sensitivities and interactions with monoamine oxidase inhibitors (MAOIs), a class of medications.

Occurrence: Tyramine is present in foods that undergo fermentation or aging processes, such as aged cheeses, fermented soy products, certain wines, and cured or smoked meats.

Biological Role: It is a trace amine and has been studied for its potential physiological effects, including its role as a neurotransmitter and its impact on blood pressure regulation.

Food Sensitivities: Some individuals may be sensitive to tyramine, and excessive intake in susceptible individuals can lead to symptoms such as headaches, increased blood pressure, and other adverse reactions.

Interaction with Medications: Tyramine is notable for its interaction with MAOIs, a class of antidepressant medications. In individuals taking MAOIs, the consumption of foods high in tyramine can lead to a hypertensive crisis.

Metabolism: In the body, tyramine is metabolized by the enzyme monoamine oxidase (MAO). Inhibition of MAO can lead to increased levels of tyramine, contributing to its potential interactions with medications.

Tyramine can be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 222 nm

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 50/50%
BufferH2SO4 – 0.1
Flow Rate1.0 ml/min
DetectionUV 222 nm
Samples 0.5 mg/ml in MeCN/H2O – 50/50%
Injection volume2 µl
LOD*28 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Biogenic amine
Analyzing CompoundsTyramine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.