HPLC Determination of Lactic Acid on Newcrom BH Column

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Lactic acid (milk acid) is a natural organic acid that plays an important role in biochemical processes.  It has a carboxylic and hydroxy- groups which, upon standing, can react with each other and form dimers, trimers and higher oligomers.  Lactic acid is a polar molecule that does not retain on reversed-phase columns. Newcrom BH mixed-mode anion-exchange column is used for the separation of lactic acid and its oligomers by a combination of weak reversed-phase and anion-exchange mechanisms. Lactates can be monitored by low UV, ELSD, CAD  or LC/MS.


Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer HClO4 – 0.1%
Flow Rate 1 ml/min
Detection UV 210 nm



Class of Compounds
Acid, Hydrophilic, Ionizable
Analyzing Compounds Lactic Acid


Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Lactic Acid
Lactic Acid Oligomers
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