HPLC Determination of Lactic Acid on Newcrom BH Column


HPLC.cloud
View on hplc.cloud

Lactic acid (milk acid) is a natural organic acid that plays an important role in biochemical processes.  It has a carboxylic and hydroxy- groups which, upon standing, can react with each other and form dimers, trimers and higher oligomers.  Lactic acid is a polar molecule that does not retain on reversed-phase columns. Newcrom BH mixed-mode anion-exchange column is used for the separation of lactic acid and its oligomers by a combination of weak reversed-phase and anion-exchange mechanisms. Lactates can be monitored by low UV, ELSD, CAD  or LC/MS.

Condition
Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer HClO4 – 0.1%
Flow Rate 1 ml/min
Detection UV 210 nm

 

Description
Class of Compounds
 Acid, Hydrophilic, Ionizable
Analyzing Compounds Lactic Acid

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Lactic Acid
Lactic Acid Oligomers
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.