HPLC Determination of HEPES on Newcrom AH Column

HPLC.cloud View on hplc.cloud HEPES is a zwitterionic organic chemical buffering agent.  It is a very polar compound which is not retained by traditional reverse phase chromatography.  HEPES can be separated by mixed-mode hydrophilic interaction chromatography on the Newcrom AH mixed-mode column, which has both hydrophobic and cationic exchange properties.  The mobile phase consists of H2O and Methanesulfonic acid.  It can be detected using a conductivity detector.


Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase H2O- 100%
Buffer Methanesulfonic acid  - 10 mM
Flow Rate 1.0 ml/min
Detection Conductivity


Class of Compounds Hydrophilic
Analyzing Compounds HEPES
Application Analytes:
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic Acid)