Ethidium bromide

Ethidium bromide structural formula

CAS Number1239-45-8
Molecular FormulaC21H20BrN3
Molecular Weight394.317
InChI KeyZMMJGEGLRURXTF-UHFFFAOYSA-N
LogP1.44
Synonyms
  • Ethidium bromide
  • 3,8-Diamino-5-ethyl-6-phenylphenanthridin-5-ium bromide
  • Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1:1)
  • 1239-45-8
  • Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide
  • 2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide
  • 2,7-Diamino-9-phenyl-10-ethylphenanthridinium bromide
  • 2,7-Diamino-9-phenylphenanthridine ethobromide
  • 3,8-diamino-1-ethyl-6-phenylphenantridinium bromide
  • 3,8-Diamino-1-ethyl-6-phenylphenantridiniumbromid
  • 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide
  • bromure de 3,8-diamino-1-ethyl-6-phenylphenantridinium
  • bromuro de 3,8-diamino-1-etil-6-fenilfenantridinio
  • Dromilac
  • Homidium bromide
  • Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1:1)
  • EINECS 214-984-6
  • Ethydium bromide
  • NSC 268986
  • Bromure d'homidium
  • Bromuro de homidio
  • Homidii bromidum
  • UNII-059NUO2Z1L
  • EtBr

Applications:

Separation of Ethidium bromide on Newcrom R1 HPLC column

May 16, 2018
Separation of Ethidium bromide on Newcrom R1 HPLC column

Ethidium bromide can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Ethidium bromide
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.