The organic acids glucuronic acid, methanesulfonic acid, and butanesulfonic acid were separated using Primesep SB mixed-mode column. Primesep SB is a reverse-phase column with embedded basic ion-pairing groups.
Column | Primesep SB, 4.6×50 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 30/70% |
Buffer | Gradient AmFm pH 3.0- 2-50 mM, 3 min, 3 min hold |
Flow Rate | 1.0 ml/min |
Detection | CAD |
Class of Compounds |
Acid, Hydrophilic, Ionizable |
Analyzing Compounds | Glucuronic acid, Methanesulfonic acid, Butanesulfonic acid |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsButanesulfonic acid
Glucuronic acid
Methanesulfonic Acid
UV Detection