HPLC Separation of Pyrilamine, Trimipramine, Pindolol Using Hydrogen Bonding Mode


Application Notes: Many drugs contain small hydrophobic and hydrophilic compounds. There are several ways to retain and analyze these compounds including, reversed-phase chromatography, cation-exchange chromatography, and HILIC. Our method includes separation based on hydrogen-bonding interactions between the analytes and the stationary phase. Hydrogen bonding offers unique selectivity of separation with good peak shape and retention control. Our method is fully compatible with ELSD, LC/MS and preparative chromatography. This approach can also be applied to the analysis of other drug molecules.

Application Columns: SHARC 1, 3.2×100 mm, 5 um, 100A. To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site.

Application Compounds: Pyrilamine, trimipramine, and pindolol

Detection Technique: UV, LC/MS


Column Sharc 1, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer AmFm, Formic acid
Flow Rate 0.5 ml/min
Detection UV, 270 nm



Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Pyrilamine, Trimipramine, Pindolol


Application Column


The SHARC™ family of innovative columns represents the first commercially available columns primarily utilizing separation based on hydrogen bonding. SHARC stands for Specific Hydrogen-bond Adsorption Resolution Column. Hydrogen bonding involves an interaction or attraction between a bound hydrogen atom and molecules containing electronegative atoms, such as oxygen, nitrogen, and fluorine.

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.