HPLC Separation of Pyrilamine, Trimipramine, Pindolol Using Hydrogen Bonding Mode

  Application Notes: Many drugs contain small hydrophobic and hydrophilic compounds. There are several ways to retain and analyze these compounds including, reversed-phase chromatography, cation-exchange chromatography, and HILIC. Our method includes separation based on hydrogen-bonding interactions between the analytes and the stationary phase. Hydrogen bonding offers unique selectivity of separation with good peak shape and retention control. Our method is fully compatible with ELSD, LC/MS and preparative chromatography. This approach can also be applied to the analysis of other drug molecules. Application Columns: SHARC 1, 3.2x100 mm, 5 um, 100A. To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site. Application Compounds: Pyrilamine, trimipramine, and pindolol Detection Technique: UV, LC/MS


Column Sharc 1, 3.2x100 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer AmFm, Formic acid
Flow Rate 0.5 ml/min
Detection UV, 270 nm


Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Pyrilamine, Trimipramine, Pindolol
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:

Application Detection:
UV Detection