one basic group

Step 6

We have established that your compound is a hydrophilic strong base with one basic group. Below is an example of a separation of a similar compound. This example can be used a starting condition in your method development.

Hydrophilic basic compounds have no or very low interaction with the stationary phase by reverse mechanism. Instead, the positive charge of the molecule can be used as a means of retention in ion-exchange mode. Several Primesep columns offer cation-exchange capabilities. The degree of retention is increased in the line of the column from Primesep C to 200 to 100 and to Primesep A.