USP Method for the Analysis of Amoxicillin using the Legacy L1 Column

  Application Notes: Amoxicillin is one of the most commonly prescribed antibiotics. It is often used for treating strep throat. According to USP methods, amoxicillin should not contain less than 900ug and no more than 1050ug of amoxicillin per mg.  The USP HPLC method for the separation of amitriptyline was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS. Application Columns: Legacy L1 C18 HPLC column Application compounds: Amoxicillin Mobile phase: MeCN/50 mM dibasic sodium phosphate Detection technique: UV Source: USP35: NF30 


Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/50 mM NaH2PO4  pH 5.0 (4/96)
Buffer NaH2PO4
Flow Rate 1.0 ml/min
Detection UV, 230 nm


Class of Compounds Drug, Antibiotics, Hydrophobic, Ionizable
Analyzing Compounds Amoxicillin
Application Analytes: