Retention of N-Nitrosarcosine on Obelisc N Column

N-Nitrocarcosine is very polar compound with LogP of -0.5. It is a nitro derivative of glycine which is slightly acidic. HPLC method was developed on Obelisc N HILIC mixed-mode HPLC column. Nitrosarcosine is retained by combination of hydrophilic interaction chromatography and anion-exchange interaction. Compound exists in two forms which can be separated by adjusting pH of the mobile phase. Method is LC/MS compatible and uses ammonium formate as mobile phase modifier.

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.