Glyphosate is a broad-spectrum herbicide which is used to kill weeds. Presence of glyphosate is strongly regulated by various governing agencies in the US, Europe, and Asia. Method for analysis of glyphosate in fruit juices and teas was developed using the Obelisc N HILIC/ion-exchange column. Glyphosate is retained by an ion-exchange mechanism. Since the method uses a highly aqueous mobile phase, which is not usual, for HILIC columns, none of the sugars, vitamins and other components of juices interfere with the analysis of glyphosate. Samples of juices were spiked with glyphosate. This method can be used in combination with LC/MS for determination of very low concentrations of glyphosate in various fruits and vegetables as well as ground and drinking water.
Obelisc N, 4.6x50 mm, 5 µm, 100A
MeCN/H2O - 20/80%
Formic acid - 0.05%
UV, 250 nm, ELSD
Class of Compounds
Herbicide, Hydrophilic, Ionizable
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