Method for Analysis of Glyphosate in Apple Juice, Cranberry Juice, Mango Juice and Green Tea

Glyphosate is a broad-spectrum herbicide which is used to kill weeds. Presence of glyphosate is strongly regulated by various governing agencies in the US, Europe, and Asia. Method for analysis of glyphosate in fruit juices and teas was developed using the Obelisc N HILIC/ion-exchange column. Glyphosate is retained by an ion-exchange mechanism. Since the method uses a highly aqueous mobile phase, which is not usual, for HILIC columns, none of the sugars, vitamins and other components of juices interfere with the analysis of glyphosate. Samples of juices were spiked with glyphosate. This method can be used in combination with LC/MS for determination of very low concentrations of glyphosate in various fruits and vegetables as well as ground and drinking water.



Column Obelisc N, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer Formic acid – 0.05%
Flow Rate 1.0 ml/min
Detection UV, 250 nm, ELSD



Class of Compounds
Herbicide, Hydrophilic, Ionizable
Analyzing Compounds Glyphosate


Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.