Method for Analysis of Glyphosate in Apple Juice, Cranberry Juice, Mango Juice and Green Tea

Glyphosate is a broad-spectrum herbicide which is used to kill weeds. Presence of glyphosate is strongly regulated by various governing agencies in the US, Europe, and Asia. Method for analysis of glyphosate in fruit juices and teas was developed using the Obelisc N HILIC/ion-exchange column. Glyphosate is retained by an ion-exchange mechanism. Since the method uses a highly aqueous mobile phase, which is not usual, for HILIC columns, none of the sugars, vitamins and other components of juices interfere with the analysis of glyphosate. Samples of juices were spiked with glyphosate. This method can be used in combination with LC/MS for determination of very low concentrations of glyphosate in various fruits and vegetables as well as ground and drinking water.  


Column Obelisc N, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer Formic acid - 0.05%
Flow Rate 1.0 ml/min
Detection UV, 250 nm, ELSD


Class of Compounds Herbicide, Hydrophilic, Ionizable
Analyzing Compounds Glyphosate
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Application Analytes:

Application Detection:
UV Detection
ELSD/MS Detection