HPLC Separation of Histamine and Phenethylamine on Primesep 200 Column

Histamine is a basic compound that acts like neurotransmitter. Phenethylamine is a natural amine alkaloid psychoactive compound with stimulant effects. It forms by enzymatic decarboxylation of phenylalanine, and also acts as neurotransmitter. Both compounds are separated in one HPLC run on Primesep C and Primesep 200 columns by reversed-phase cation-exchange mechanism. Primesep C provides shorter retention time and histamine and phenethylamine can be separated within 5 minutes. Primesep C and Primesep 200 columns do not require ion-pairing reagent as it is attached to the surface of Primesep silica gel. Method can be used for analysis of these two compounds in plasma, blood and urine with UV, ELSD, CAD or LC/MS detection.

Application Column

Primesep 200

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart

Histamine
Phenethylamine

UV Detection

SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Alltesta™ Cromite Analyzer

The Cromite™ Analyzer represents a new generation of affordable and easy-to-learn HPLC instrumentation for schools, colleges and universities.

With automated single-vial injection, high-pressure flow and UV/Vis detection capabilities, scholars can use this instrument to separate compounds, such as vitamins in juices, food dyes, caffeine and active ingredients in pharmaceutical products. The cloud-connected software allows you to run tests, analyze results and share data from anywhere and with any device. SIELC Technologies provides a vast library of free methods for a variety of compounds, enabling students to characterize and understand the chemical composition of many different products. The Cromite™ Analyzer comes with a column of your choice and all necessary accessories to perform multiple analyses. For pricing information, please contact us at

Alltesta™ Gradient Automated Analyzer

SIELC's Alltesta™ Analyzer is an affordable HPLC analytical system that provides both scientific and educational environments with a convenient, simple solution for measuring small and trace concentrations of various chemicals in products such as milk, baby formula, pet food, pills, household formulations, and more. For pricing information, please contact us at

Alltesta™ Mini Autosampler

Based on a proprietary alpha/beta mechanic configuration, this miniature autosampler offers inexpensive and reliable automation for many analytical- and liquid-handling settings, including HPLC. Direct and comprehensive control of this small autosampler’s features, as well as high-level commands for succinct automation, provide quick and easy integration. Users can customize their mini autosampler with various valve configurations, tray designs, and syringe volumes and pressures. A built-in shaking feature allows for sample-mixing before injection and/or simple sample extraction inside the vial. The price of the Alltesta™ Mini Autosampler varies depending on the specific configuration requested. For more information on pricing, please contact us at