HPLC Separation of Epinephrine on Legacy L1 Column

Epinephrine was analyzed according to the US Pharmacopeia method on traditional reversed-phase HPLC columns, classified as a L1 type of column according to USP classification. Legacy L1 column can be used for the analysis of drug substances in various formulations. Legacy L1 can be used in new HPLC methods as well as in validated methods which require experiments based on USP manuscript. The mobile phase can be modified to accommodate other detection techniques, like LC/MS.


Column Legacy L1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeOH – 15%
Buffer Sodium 1 -Octane sulfonate/EDTA
Flow Rate 1.0 ml/min
Detection UV, 280 nm



Class of Compounds
Drug, Neurotransmitter, Hydrophobic, Ionizable
Analyzing Compounds Epinephrine


Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.