HPLC Separation of Epinephrine on Legacy L1 Column

Epinephrine was analyzed according to the US Pharmacopeia method on traditional reversed-phase HPLC columns, classified as a L1 type of column according to USP classification. Legacy L1 column can be used for the analysis of drug substances in various formulations. Legacy L1 can be used in new HPLC methods as well as in validated methods which require experiments based on USP manuscript. The mobile phase can be modified to accommodate other detection techniques, like LC/MS.


Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeOH - 15%
Buffer Sodium 1 -Octane sulfonate/EDTA
Flow Rate 1.0 ml/min
Detection UV, 280 nm


Class of Compounds Drug, Neurotransmitter, Hydrophobic, Ionizable
Analyzing Compounds Epinephrine
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Application Analytes:

Application Detection:
UV Detection