HPLC Separation of Aspartic Acid and Asparagine on Obelisc R Column

Two amino acids, aspartic acid and asparagine, are separated on an Obelisc R column by combination of reverse-phase and ion-exchange mechanism. Method can be used for analysis of these and other underivatized amino acids by HPLC with UV, ELSD, CAD and LC/MS detection.


Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm, AmAc
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Asparagine, Aspartic Acid

Application Analytes:
Aspartic Acid