Effect of mobile phase composition on retention of 3 compounds on Obelisc R

This application shows the effect of mobile phase composition on retention of acidic, basic and neutral compound. Retention of basic and acidic compounds is controlled by buffer pH and concentration, while retention of neutral compound is controlled by the amount of acetonitrile. Buffer pH changes not only ionization state of ionizable basic and acidic compounds, but ionization state of trimodal stationary phase Obelisc R. Available detection techniques include UV, ELSD, LC/MS, and Corona.


Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc
Flow Rate 1.0 ml/min
Detection 250


Class of Compounds Acid, Hydrophilic, Neutral, BasicIonizable
Analyzing Compounds Benzonitrile, Benzoic Acid, Benzylamine
Application Analytes:
Benzoic Acid