Analysis of Antibiotic Tylosin and Related Impurities in Mixed-Mode Chromatography

Tylosin is a macrolide antibiotic with a broad spectrum of activity against gram-negative and gram-positive organisms. It is highly polar and basic molecule with limited retention in reversed-phase chromatography. Tylosin and related impurities were analysed on Obelisc R trimodal column. Method is compatible with LC/MS and prep chromatography and can be used for HPLC analysis of tylosin in various biofluids.  


Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-30%, 10 min,  5 min hold
Buffer Gradient Formic Acid - 0.05%-0.3%, 10 min,  5 min hold
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Tylosin
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Application Analytes:

Application Detection:
UV Detection
ELSD/MS Detection