Vitamin B5 (Pantothenic Acid)

Vitamin B5 (Pantothenic Acid) structural formula

CAS Number79-83-4
Molecular FormulaC9H17NO5
Molecular Weight219.237
InChI KeyGHOKWGTUZJEAQD-ZETCQYMHSA-N
LogP-1.32
Synonyms
  • Pantothenic acid
  • N-[(2R)-2,4-Dihydroxy-3,3-dimethylbutanoyl]-beta-alanine
  • beta-Alanine, N-[(2R)-2,4-dihydroxy-3,3-dimethyl-1-oxobutyl]-
  • 79-83-4
  • D-Pantothenic acid
  • 4-04-00-02569
  • β-Alanine, N-[(2R)-2,4-dihydroxy-3,3-dimethyl-1-oxobutyl]-
  • (+)-Pantothenic acid
  • (D)-(+)-Pantothenic acid
  • acide D-pantothenique
  • acido D-pantotenico
  • Chick antidermatitis factor
  • D(+)-N-(2,4-Dihydroxy-3,3-dimethylbutyryl)-β-alanine
  • D-Pantothensaure
  • Pantothenic acid, D-
  • β-Alanine, N-(2,4-dihydroxy-3,3-dimethyl-1-oxobutyl)-, (R)-
  • BRN 1727064
  • (D,+)-N(alpha-gamma-Dihydroxy-beta,beta-dimethylbutyryl)-beta-alanine
  • D(+)-N-(2,4-Dihydroxy-3,3-dimethylbutyryl)-beta-alanine
  • N-(2,4-Dihydroxy-3,3-dimethylbutyryl)-beta-alanine
  • EINECS 201-229-0
  • Kyselina pantothenova
  • UNII-19F5HK2737
  • 3-[[(2S)-2,4-dihydroxy-3,3-dimethyl-1-oxo-butyl]amino]propanoic acid
  • 3-[[(2S)-2,4-dihydroxy-3,3-dimethyl-butanoyl]amino]propanoic acid
  • D-(+)-pantothenic acid
  • (R)-pantothenic acid

Applications:

HPLC Method for Separation of Vitamins Group B such as Nicotinic Acid, Pyridoxine, Niacinamide, Pantothenic Acid, Riboflavin on Obelisc N Column

August 22, 2008

Separation of vitamins group B is achieved on Obelisc N column in HILIC mixed-mode. Vitamins of this group are different in polarity and ionic properties. Retention and separation is achieved by optimization of amount of ACN, buffer and buffer pH. Combination of UV and ELSD detection is used to monitor HPLC separation. B vitamins are water-soluble vitamins that play an important role in cell metabolism. Supplements containing all six are generally referred to as a vitamin B complex. Individual B vitamin supplements are referred to by the specific name of each vitamin. This method can be used to analyze individual B vitamins as well as vitamin B complex. Isolation of impurities as well as degradation products is possible by preparative chromatography.

Condition
Column Obelisc N , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAC pH 4.5 – 10 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm, ELSD

 

Description
Class of Compounds
 Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Pyridoxine,  Niacinamide, Pantothenic acid, Riboflavin

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Niacinamide
Vitamin B1 (Thiamine)
Vitamin B2 (Riboflavin)
Vitamin B3 (Niacin)
Vitamin B5 (Pantothenic Acid)
Vitamin B6 (Pyridoxine)
Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.