Epinephrine Sulfonate

Epinephrine Sulfonate

Synonyms

    Applications:

    HPLC Analysis of Epinephrine and Epinephrine Sulfonate on Primesep AB Column

    May 12, 2015

     

    Epinephrine and epinephrine sulfonate were analyzed using Primesep AB, a reverse-phase column with both acidic and basic embedded ion-pairing groups. This method is LC/MS compatible.

     

    Condition

    Column Primesep AB, 4.6×250 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 5/95%
    Buffer H3PO4 – 0.25%
    Flow Rate 1.0 ml/min
    Detection UV, 280 nm

     

    Description

    Class of Compounds
    Drug, Hydrophilic, Ionizable, Vitamin, Supplements
    Analyzing Compounds Epinephrine, Epinephrine Sulfonate

     

    Application Column

    Primesep AB

    Column Diameter: 4.6 mm
    Column Length: 250 mm
    Particle Size: 5 µm
    Pore Size: 100 A

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    Application Analytes:
    Epinephrine
    Epinephrine Sulfonate

    Application Detection:
    ELSD Detection
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Separation of Epinephrine and Epinephrine Sulfonate

    June 5, 2011


    Epinephrine and epinephrine sulfonate are separated on combination of Primesep 100 and Primesep SB HPLC columns. Primesep 100 column retains epinephrine by combination of reversed-phase and cation-exchange mechanisms. Epinephrine sulfonate is retained by weak reversed-phase mechanism. Due to strong zwitter-ionic nature of epinephrine sulfonate, ionic interaction is not available for this molecule. Compounds can be monitored by UV, ELSD, CAD or LC/MS.

    Condition

    Column Primesep SB, Primesep 100 4.6×250 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O – 70/30%
    Buffer AmFm
    Flow Rate 1.0 ml/min
    Detection UV, 270 nm

     

    Description

    Class of Compounds
    Drug, Hydrophilic, Ionizable, Vitamin, Supplements
    Analyzing Compounds Epinephrine, Epinephrine Sulfonate

     

    Application Column

    Primesep 100

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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    Primesep SB

    The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

    Select options
    Application Analytes:
    Epinephrine
    Epinephrine Sulfonate

    Application Detection:
    ELSD Detection
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HILIC Separation of Epinephrine and Epinephrine Sulfonate

    August 22, 2008

    Epinephrine (also referred to as adrenaline) is a hormone and neurotransmitter. It is a catecholamine, a sympathomimetic monoamine derived from the amino acids phenylalanine and tyrosine. This method can be used to determine and quantitate epinephrine and epinephrine sulfonate in biological fluids (urine, blood, serum) and drug formulations. Obelisc N columns are used to retain and separate epinephrine and epinephrine sulfonate in mixed-mode hydrophilic interaction chromatography. Epinephrine is retained by the combination of cation-exchange and HILIC mechanisms. Epinephrine sulfonate is retained by HILIC mechanism. Buffer concentration and pH, as well as the amount of acetonitrile, can be used to adjust retention of both compounds. Both compounds can be detected by UV, ELSD and LC/MS. Preparative separation is possible with volatile mobile phases (ammonium formate or ammonium acetate.

    Condition

    Column Obelisc N, 4.6×150 mm, 5 µm, 100A
    Mobile Phase MeCN/H2O
    Buffer AmFm pH 3.0
    Flow Rate 1.0 ml/min
    Detection UV, 270 nm

     

    Description

    Class of Compounds
    Drug, Hydrophilic, Ionizable, Vitamin, Supplements
    Analyzing Compounds Epinephrine, Epinephrine Sulfonate

     

    Application Column

    Obelisc N

    SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

    Select options
    Application Analytes:
    Epinephrine
    Epinephrine Sulfonate

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.