Alginic acid is a linear copolymer with homopolymeric blocks of (1-4)-linked ?-D-mannuronate (M) and its C-5 epimer ?-L-guluronate (G) residues, respectively, covalently linked together in different sequences or blocks. The monomers can appear in homopolymeric blocks of consecutive G-residues (G-blocks), consecutive M-residues (M-blocks), alternating M and G-residues (MG-blocks), or randomly organized blocks. Molecular weight varies from 10,000 to 600,000. Analysis is a challenging task due to a wide distribution of molecular weights. Alginic acid analyzed in ion-exclusion and size exclusion mode on Primesep C mixed-mode cation exchange column. Alginic acid elutes before void as a single peak with good symmetry. Method can be used for quantitation of alginic acid in various formulation. Low UV or ELSD can be employed.
Application Column
Primesep C
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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