HPLC Method For Analysis Of Meldonium in Mildronate Tablet Dosage Form on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis of L-Carnitine and Arginine

Meldonium is a popular treatment for ischemia in Eastern Europe. It helps open the arteries, thereby increasing blood flow. Due to reports of athletes using it as a performance-enhancing drug, it has been banned by the World Anti-Doping Agency since 2016.
Meldonium can be detected in the low UV regime. Using a Primesep 100 reverse-phase column and a mobile phase consisting of water and acetonitrile (MeCN) with a perchloric acid (HClO4) buffer, meldonium can be retained, measured, and analyzed. This analysis method can be UV detected at 205 nm.

Condition
Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 25/75%
Buffer HClO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV, 205 nm

 

Description
Class of Compounds
 Amino acid, Drug
Analyzing Compounds Meldonium

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Meldonium
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.