Dexamethasone

Dexamethasone structural formula

CAS Number50-02-2
Molecular FormulaC22H29FO5
Molecular Weight392.467
InChI KeyUREBDLICKHMUKA-CXSFZGCWSA-N
LogP1.83
Synonyms
  • Dexamethasone
  • (11beta,16alpha)-9-Fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3,20-dione
  • Pregna-1,4-diene-3,20-dione, 9-fluoro-11,17,21-trihydroxy-16-methyl-, (11beta,16alpha)-
  • 50-02-2
  • Pregna-1,4-diene-3,20-dione, 9-fluoro-11,17,21-trihydroxy-16-methyl-, (11beta,16alpha)-
  • (11beta,16alpha)-9-Fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3,20-dione
  • 16alpha-Methyl-9alpha-fluoro-1,4-pregnadiene-11beta,17alpha,21-triol-3,20-dione
  • 16alpha-Methyl-9alpha-fluoro-11beta,17alpha,21-trihydroxypregna-1,4-diene-3,20-dione
  • 16alpha-Methyl-9alpha-fluoroprednisolone
  • 16alpha-Methyl-9alpha-fluoro-delta1-hydrocortisone
  • 1-Dehydro-16alpha-methyl-9alpha-fluorohydrocortisone
  • 9-Fluoro-11beta,17,21-trihydroxy-16alpha-methylpregna-1,4-diene-3,20-dione
  • 9alpha-Fluoro-11beta,17alpha,21-trihydroxy-16alpha-methyl-1,4-pregnadiene-3,20-dione
  • 9alpha-Fluoro-16alpha-methyl-1,4-pregnadiene-11beta,17alpha,21-triol-3,20-dione
  • 9alpha-Fluoro-16alpha-methyl-11beta,17,21-trihydroxypregna-1,4-diene-3,20-dione
  • 9alpha-Fluoro-16alpha-methylprednisolone
  • Adexone
  • Aeroseb-Dex
  • Aphtasolon
  • Aphthasolone
  • Calonat
  • Corsone
  • Cortisumman
  • Decacort
  • Decaderm
  • Decadron A
  • Decalix
  • Decasone
  • Dekacort
  • Delipos
  • Deltafluorene
  • Dergramin
  • Deronil
  • Desadrene
  • Desameton
  • Deseronil
  • Dexacort
  • Dexacortal
  • Dexa-Cortidelt
  • Dexacortin
  • Dexadeltone
  • Dexafarma
  • Dexalona
  • Dexaltin
  • Dexa-Mamallet
  • dexametasona
  • Dexameth
  • Dexamethason
  • Dexamethasone alcohol
  • Dexamonozon
  • Dexapolcort
  • Dexapos
  • Dexaprol
  • Dexa-Scheroson
  • Dexa-sine
  • Dexason
  • Dexasone
  • Dexinoral
  • Dexonium
  • Dextelan
  • Dinormon
  • Etacortilen
  • Fluormone
  • Fluorocort
  • Gammacorten
  • Gentalipos
  • Hexadecadrol
  • Hexadrol
  • Isopto-Dex
  • Lokalison F
  • Loverine
  • Luxazone
  • Maxidex
  • Millicorten
  • NSC 34521
  • Oradexon
  • Pet-Derm III
  • Prednisolon F
  • Prednisolone F
  • Pregna-1,4-diene-3,20-dione, 9-fluoro-11beta,17,21-trihydroxy-16alpha-methyl-
  • Superprednol
  • Surodex
  • Visumetazone
  • Aeroseb-D
  • Anaflogistico
  • Auxiron
  • Bisu DS
  • Decacortin
  • Decadron
  • Decaspray
  • Dectancyl
  • Desametasone
  • Desamethasone
  • Dexa Mamallet
  • Dexa-Cortisyl
  • Dex-ide
  • Dexinolon
  • EINECS 200-003-9
  • Fluormethylprednisolone
  • delta1-9alpha-Fluoro-16alpha-methylcortisol
  • 4-alpha-Fluoro-16-alpha-methyl-11-beta,17,21-trihydroxypregna-1,4-diene-3,20-dione
  • Mediamethasone
  • 16alpha-Methyl-9alpha-fluoro-1-dehydrocortisol
  • 16-alpha-Methyl-9-alpha-fluoro-1-dehydrocortisol
  • 16-alpha-Methyl-9-alpha-fluoroprednisolone
  • 16alpha-Methyl-9alpha-fluoro-delta(sup 1)-hydrocortisone
  • 16-alpha-Methyl-9-alpha-fluoro-delta(sup 1)-hydrocortisone
  • 16-alpha-Methyl-9-alpha-fluoro-11-beta,17-alpha,21-trihydroxypregna-1,4-diene-3,20-dione
  • Mexidex
  • Ocu-trol
  • Pet Derm III
  • Policort
  • Prednisolone, 9alpha-fluoro-16alpha-methyl-
  • SK-Dexamethasone
  • Spoloven
  • Sunia Sol D
  • delta(sup 1)-9-alpha-Fluoro-16-alpha-methylcortisol
  • Dexamethasone Intensol
  • Dexone 0.75
  • Mymethasone
  • Decaject
  • Decaject-L.A.
  • Decameth
  • Methylfluorprednisolone
  • Dexamethasonum
  • UNII-7S5I7G3JQL
  • Ozurdex
  • 1050677-47-8
  • 137098-19-2
  • 8054-59-9
  • 906362-70-7
  • 906422-84-2

Applications:

UV-Vis Spectrum of Dexamethasone

July 19, 2024
UV-Vis Spectrum of Dexamethasone. Absorption Maxima: 240 nm.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Dexamethasone
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method of Analysis for Dexamethasone and Prednisolone

June 23, 2020


HPLC.cloud
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Dexamethasone is a corticosteroid that is used as a replacement of the natural hormone produced by adrenal glands when the body can’t produce enough on its own.  It has also been found to reduce deaths among the critically ill patients with the COVID-19.  Prednisolone is another corticosteroid used to treat a variety of inflammatory conditions and has a similar structure to dexamethasone.  Both steroids can be retained and separated in HPLC using Newcrom A mixed-mode column with the mobile phase consisting of either acetonitrile (ACN) or methanol (MeOH) and water with formic acid buffer.  Methanol offers longer retention time compared to acetonitrile.  UV detection at 275nm.

Condition

Column Newcrom A , 3.2×100 mm, 3 µm, 100A
Mobile Phase MeCN, MeOH
Buffer Formic Acid  – 0.2%
Flow Rate 0.5 ml/min
Detection UV, 275 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable
Analyzing Compounds Dexamethasone, Prednisolone

 

Application Column

Newcrom A

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Dexamethasone
Prednisolone
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Dexamethasone on Newcrom R1 HPLC column

February 16, 2018
Separation of Dexamethasone on Newcrom C18 HPLC column

Dexamethasone is a corticosteroid used to treat rheumatic problems, skin diseases, and allergies, and others. It works by stimulating the glucocorticoid receptor. Dexamethasone can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Dexamethasone
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.

HPLC Method for Analysis of Sulfadiazine, Naphthylthiourea, Sulfamethazine, Dexamethasone, Cortisone 21-Acetate

July 11, 2017

Sulfadiazine is an antibiotic used to prevent rheumatic fever, chancroid, chlamydia and infections by Haemophilus influenzae. There are side effects to the use of the drug which include, but not limited to: nausea, headache, rash, depression.

 

Condition

Column Primesep 100, 3.2×50 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 20-75%, 4 min
Buffer Gradient H3PO4 – 0.05-0.2%, 4 min
Flow Rate 0.5 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable
Analyzing Compounds Sulfadiazine, Naphthylthiourea, Sulfamethazine, Dexamethasone, Cortisone 21-Acetate

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Cortisone 21-Acetate
Dexamethasone
Naphthylthiourea
Sulfadiazine
Sulfamethazine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.