USP Methods for the Analysis of Procainamide

  Application Notes: Procainamide is a common antiarrhythmic agent used to treat cardiac arrhythmias. According to USP methods procainamide hydrochloride contains not less than 98% and no more than 102% procainamide based on the dried basis. The USP HPLC method for the separation of procainamide and 4-aminobenzoic was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS. Reference: USP 35- NF30


Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O - 60/40%
Buffer TEAPh pH 7.5
Flow Rate 1.0 ml/min
Detection UV, 270 nm


Class of Compounds Drug, Antiarrhythmic, Hydrophobic, Ionizable
Analyzing Compounds Procainamide, 4-Aminobenzoic acid
Application Analytes:
4-Aminobenzoic Acid