USP Methods for the Analysis of Ibuprofen Using Legacy L1 Column

  Application Notes: Ibuprofen is the most commonly used analgesic. It is a vasoconstrictor and fever reducer. According to the USP methods, ibuprofen contains no less than 97% and no more than 103% ibuprofen based on a dried basis. The USP HPLC method for the separation of ibuprofen was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP. Application Columns: Legacy L1 C18 HPLC column Application compounds: Ibuprofen and benzophenone Mobile phase: .01M H3PO4/MeCN (55/45) Detection technique: UV Reference: USP35: NF30


Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase .01M H3PO4 /MeCN (45/55)
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection UV, 254 nm


Class of Compounds Drug,  Hydrophobic, Ionizable
Analyzing Compounds Ibuprofen, Benzophenone
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Application Analytes:

Application Detection:
UV Detection