USP Methods for Nitrofurantoin for the Legacy L1 Column

USP Methods for Nitrofurantoin for the Legacy L1 Column   Application Notes: Nitrofurantoin is an antibiotic used to treat urinary tract infections and E. coli. The USP HPLC method for the separation of nitrofuranton was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS. Application Columns: Legacy L1 C18 HPLC column Application compounds: Nitrofurantoin Mobile phase: NaH2PO4 pH 7.0/MeCN 88/12 Detection technique: UV Reference: USP35: NF30


Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase : NaH2PO4 pH 7.0/MeCN 88/12
Buffer NaH2PO4
Flow Rate 1.0 ml/min
Detection UV, 254 nm


Class of Compounds Drug, Antibiotics, Hydrophobic, Ionizable
Analyzing Compounds Nitrofurantoin
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Application Analytes:

Application Detection:
UV Detection