Separation of Trisaccharides on SIELC Columns

 

Trisaccharides can be difficult to separate using conventional HPLC columns. The four trisaccharides panose, raffinose, isomaltotriose, and kestose have the same chemical formula, and the all have two glycosidic bonds connecting the three monosaccharides. Obelisc N was used as a stationary phase to separate trisaccharides because it is capable of multiple modes of separation. Obelisc N is a highly polar column that retains polar and charged analytes. Even though the trisaccharides differ only in regio- and stereochemistry they are resolved.

Condition 

Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 75%
Buffer AmFm pH 3.0- 10 mM
Flow Rate 1.0 ml/min
Detection ELCD

Description

Class of Compounds
Trisaccharides,  Hydrophilic, Ionizable
Analyzing Compounds Raffinose, Kestose, Panose, Isomaltose

 

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Isomaltotriose
Kestose
Panose
Raffinose

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.