Diphenhydramine

Diphenhydramine

CAS Number58-73-1
Molecular FormulaC17H21NO
Molecular Weight255.35
InChI Key3.3
Synonyms
  • 2-Diphenylmethoxy-N,N-dimethylethylamine
  • Allerdryl
  • Benadryl
  • Benhydramin
  • Benylin
  • Benzhydramine
  • Citrate, Diphenhydramine
  • Dimedrol
  • Diphenhydramine
  • Diphenhydramine Citrate
  • Diphenhydramine Citrate (1:1)
  • Diphenhydramine Hydrochloride
  • Diphenylhydramin
  • Diphenylhydramine
  • Dormin
  • Hydrochloride, Diphenhydramine

Applications:

Separation of Diphenhydramine hydrochloride on Newcrom R1 HPLC column

February 16, 2018
Separation of Diphenhydramine hydrochloride on Newcrom C18 HPLC column

Diphenhydramine hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Diphenhydramine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.

HPLC Separation of Acetaminophen, Phenylephrine, and Diphenhydramine

March 3, 2006

Primesep C separates acetaminophen (paracetamol), phenylephrine, and diphenhydramine– common over-the-counter cold and flu medication ingredients such as Tylenol products—by either an acetonitrile or phosphoric acid gradient. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and phosphoric acid with ultraviolet (UV) detection at 210 nm.

Condition
Column Primesep C, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

 

Description
Class of Compounds
 Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Pseudoephedrine, Diphenhydramine

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Acetaminophen (Paracetamol)
Diphenhydramine
Phenylephrine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.