Aconitic acid

CAS Number209-564-4
Molecular FormulaC6H6O6
Molecular Weight174.11
InChI KeyGTZCVFVGUGFEME-IWQZZHSRSA-N
LogP-1
Synonyms
  • cis-Aconitic acid
  • 585-84-2
  • cis-aconitate
  • ACONITIC ACID
  • Achilleic acid
  • Citridinic acid
  • Equisetic acid
  • Pyrocitric acid
  • 1-Propene-1,2,3-tricarboxylic acid
  • Achilleaic acid
  • Citridic acid
  • (Z)-prop-1-ene-1,2,3-tricarboxylic acid
  • (1Z)-prop-1-ene-1,2,3-tricarboxylic acid
  • cis-Aconic acid
  • (Z)-Aconitic acid
  • cis-Oxaloacetic acid
  • (Z)-1-Propene-1,2,3-tricarboxylic acid
  • 499-12-7
  • Glutaconic acid, 3-carboxy-
  • 2-Pentenedioic acid, 3-carboxy-
  • UNII-OF5471ZHRR
  • cis-1-propene-1,2,3-tricarboxylic acid
  • 1-Propene-1,2,3-tricarboxylic acid, (1Z)-
  • MFCD00063184
  • Propene-1,2,3-tricarboxylic acid
  • OF5471ZHRR
  • CHEMBL347285
  • 1-Propene-1,2,3-tricarboxylic acid, cis-
  • CHEBI:32805
  • propene-1,2,3-tricarboxylicacid
  • 3-Carboxy-2-pentenedioic acid
  • 3-Carboxyglutaconic acid
  • cis-Propene-1,2,3-tricarboxylic acid
  • NSC7616
  • cis-Aconitum acid
  • FEMA No. 2010
  • Aconitic acid, cis-
  • CCRIS 2507
  • HSDB 635
  • Aconitic acid, (Z)-
  • EINECS 207-877-0
  • 1-Propene-1,3-tricarboxylic acid
  • NSC-7616
  • cis-Aconate

Applications:

HPLC Method for Analysis of Aconitic Acid on BIST A+

November 30, 2022

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Aconitic Acid on BIST A Column by SIELC Technologies

HPLC Method for Analysis of Aconitic Acid on BIST A+ by SIELC Technologies
HPLC Method for Analysis of Aconitic Acid on BIST A+

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Aconitic Acid

Aconitic acid is a popular compound used to artificial create nutty flavors in foods. It is also a common intermediate in the Kreb’s cycle when Citrate is changed into D-isocitrate. Using SIELC’s newly introduced BIST™ method, Aconitic acidcan be retained easily on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include DMP, Calcium acetate, and Magnesium acetate. Using this new and unique analysis method, Aconitic acid can be retained with high selectivity and great peak shape. This method can be detected and is compatible with ELSD, CAD, and Mass Spectrometry (LC-MS).

Condition

ColumnBIST™ A+, 2.1×100 mm, 5 µm, 100A
Mobile PhaseMeCN – 50/50%
BufferTMDAP formate pH 5.0 – 5,0 mM
Flow Rate0.5 ml/min
DetectionMS, ESI-, m/z = 173

Description

Class of CompoundsAcid, Carboxylic acids, Tricarboxylic acids
Analyzing CompoundsAconitic Acid

Application Column

BIST A

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options

BIST A+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
Aconitic acid
trans-Aconitic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Aconitic Acid on BIST A+ Column

October 15, 2022

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies


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HPLC Analysis of Aconitic Acid on BIST A+ Column
HPLC Method for Analysis of Aconitic Acid on BIST A+ Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Aconitic Acid

Aconitic acid is a popular compound used to artificial create nutty flavors in foods. It is also a common intermediate in the Kreb’s cycle when Citrate is changed into D-isocitrate. Using SIELC’s newly introduced BIST™ method, Aconitic acidcan be retained easily on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include DMP, Calcium acetate, and Magnesium acetate. Using this new and unique analysis method, Aconitic acid can be retained with high selectivity and great peak shape. This method can be detected and is compatible with ELSD, CAD, and Mass Spectrometry (LC-MS).

Application Column

BIST A

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options

BIST A+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
Aconitic acid
trans-Aconitic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Maleic  Acid, Nicotinic Acid, Aconitic Acid and Fumaric Acid on BIST™ A+ Column

July 7, 2022

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Organic Acids on BIST™ A+ Column
High Performance Liquid Chromatography (HPLC) Method for Analysis of  Maleic  Acid, Nicotinic Acid, Aconitic Acid, Fumaric Acid

The maleate ion from Maleic acid is a popular ingredient as the maleate salt in several different drugs, including Methergine, Pyrilamine, and Carfenazine, among others. Nicotinic acid, also known as Niacin or Vitamin B3, is an essential nutrient for the human body and is sometimes taken as a treatment for high cholesterol. Aconitic acid is a key intermediary in the citric acid cycle, and is also used a flavoring agent and in the production of rubbers and plastics. Fumaric acid is a popular preservative and food additive with a fruit-like taste. Using SIELC’s newly introduced BIST™ method, a mixture of these organic acids can be separated on a negatively-charged, cation-exchange BIST™ A+ column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes.

Condition

ColumnBIST™ A+, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 70%
BufferTMDAP ( N,N,N’,N’-Tetramethyl-1,3-diaminopropane) Phosphate  – 10 mM pH 4.0
Flow Rate1.0 ml/min
DetectionUV 200 nm

Description

Class of Compounds
Acid, Dicarboxylic acid, Tricarboxylic acid, Pyridinecarboxylic acid
Analyzing CompoundsMaleic  Acid, Nicotinic Acid, Aconitic Acid, Fumaric Acid

Application Column

BIST A+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
Aconitic acid
Fumaric Acid
Maleic Acid
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)
trans-Aconitic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.