Tartrazine (FD&C Yellow 5)

Tartrazine structural formula

CAS Number1934-21-0
Molecular FormulaC16H9N4Na3O9S2
Molecular Weight534.360
InChI KeyZLWLTDZLUVBSRJ-GLCFPVLVSA-K
LogP-0.479
Synonyms
  • FD&C Yellow 5
  • Trisodium 5-oxo-1-(4-sulfonatophenyl)-4-[(E)-(4-sulfonatophenyl)diazenyl]-2,5-dihydro-1H-pyrazole-3-carboxylate
  • 1H-Pyrazole-3-carboxylic acid, 2,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(E)-2-(4-sulfophenyl)diazenyl]-, sodium salt (1:3)
  • 1934-21-0
  • 1H-Pyrazole-3-carboxylic acid, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-, trisodium salt
  • C.I. 19140
  • Tartrazine
  • 1-(4-SULFOPHENYL)-3-CARBOXY-4-(4- SULFOPHENYL)AZO-5-PYRAZOLONE, TRISODIUM SALT
  • 1310 Yellow
  • 1409 Yellow
  • 1H-Pyrazole-3-carboxylic acid, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[(sulfophenyl)azo]-, trisodium salt
  • 1H-Pyrazole-3-carboxylic acid, 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-[2-(4-sulfophenyl)diazenyl]-, sodium salt (1:3)
  • 3-Carboxy-5-hydroxy-1-p-sulfophenyl-4-p-sulfophenylazopyrazole trisodium salt
  • 4,5-Dihydro-5-oxo-1-(4-sulfophenyl)-4-[(4-sulfophenyl)azo]-1H-pyrazole-3-carboxylic acid, trisodium salt
  • 5-hidroxi-1-(4-sulfofenil)-4-(4-sulfofenilazo)pirazol-3-carboxilato de trisodio
  • 5-Hydroxy-1-(4-sulfophenyl)-4-(4-sulfophenylazo)pyrazole-3-carboxylate de trisodium
  • 5-Hydroxy-1-p-sulfophenyl-4-(p-sulfophenylazo)pyrazole-3-carboxylic acid, trisodium salt
  • A.F. Yellow No.4
  • Acid Yellow 23
  • Acid Yellow N
  • Acid Yellow T
  • Acid Yellow XX-SF
  • Acilan Yellow GG
  • Airedale Yellow T
  • Aizen Tartrazine
  • Amacid Yellow T
  • Amacid Yellow T-EX
  • Apollo Acid Tartrazine
  • Atul Tartrazine
  • Basovit Yellow 133E
  • Bosovit Yellow 133E
  • Bucacid Tartrazine
  • C.I. Acid Yellow 023
  • C.I. ACID YELLOW 23
  • C.I. Acid Yellow 23, trisodium salt
  • C.I. Food Yellow 4
  • C.I. Solvent Yellow 57
  • Calcocid Yellow MCG
  • Calcocid Yellow XX
  • Canacert Tartrazine
  • Certicol Tartrazol Yellow S
  • Cilefa Yellow T
  • Cogilor Yellow 113.11
  • Cogilor Yellow 113.11-90003
  • Curon Fast Yellow 5G
  • D and C Yellow No. 5
  • DC Yellow 5
  • Dinacid Tartarzine Yellow PFD
  • Dolkwal Tartrazine
  • Duasyn Acid Yellow XX
  • Duasyn Acid Yellow XX-SF
  • Duasyn Acid Yellow XX-SF-LP 413
  • Durkee Yellow Food Color
  • Dyacid Yellow N
  • Dye Yellow Lake
  • Dynacid Yellow K
  • Edicol Supra Tartrazine N
  • Egacid Yellow T
  • Egg Yellow A
  • Eniacid Tartazine J
  • Erio Tartrazine
  • Erio Yellow T Supra
  • Eurocert Tartrazine
  • Eurocert Tartrazine 311840
  • FD & C Yellow No. 5-307008
  • FD AND C YELLOW NO. 5
  • FD&C Yellow #5 dye
  • FD&C Yellow No. 5
  • FD&C Yellow No. 5-37000
  • FD&C Yellow No. 5-37009
  • FD&C Yellow No. 5-90123
  • Fenazo Yellow T
  • Food Dye Yellow 4
  • Food dye yellow number 4
  • Food Yellow 4
  • Food Yellow No. 4
  • HD Tartrazine
  • HD Tartrazine Supra
  • Hexacert Yellow No. 5
  • Hexacol Tartrazine
  • Hidazid Tartrazine
  • Hispacid Fast Yellow T
  • Hydrazine yellow
  • Hydroxine Yellow L
  • Kako Tartrazine
  • Kayaku Food Colour Yellow No. 4
  • Kayaku Tartrazine
  • KCA Foodcol Tartrazine PF
  • KCA Tartrazine PF
  • Kemacid Tartrazine NS
  • Kiton Yellow T
  • L Yellow Z 1020
  • Lake Yellow
  • Lemon Yellow A
  • Lemon Yellow A Geigy
  • Maple Tartrazol Yellow
  • Mitsui Tartrazine
  • Multacid Yellow T
  • Naphtocard Yellow O
  • Necol Tartrazine
  • Neelicol Tartrazine
  • Neklacid Yellow T
  • NSC 4760
  • Orient Water Yellow 1
  • Oxanal Yellow T
  • Pacid Tartrazin
  • Pro-Jet Yellow OAM
  • PuriColor Yellow AYE 23
  • Ritacid Yellow NS
  • Romexal Yellow T
  • Sandolan Yellow E-TZ
  • San-ei Tartrazine
  • Sicovit Tartazin Lake E 102
  • Sicovit Tartrazine Lake E 102
  • Solvent Yellow 57
  • Sugai Tartrazine
  • Tartar Yellow FS
  • Tartar Yellow N
  • Tartar Yellow PF
  • Tartar Yellow S
  • Tartran Yellow
  • Tartraphenine
  • Tartrazin
  • Tartrazine 307043
  • Tartrazine 36008
  • Tartrazine 37000
  • Tartrazine 37009
  • Tartrazine 90123
  • Tartrazine A
  • Tartrazine A Export
  • Tartrazine B
  • Tartrazine B.P.C.
  • Tartrazine C
  • Tartrazine E 102
  • Tartrazine FQ
  • Tartrazine G
  • Tartrazine GR
  • Tartrazine Lake
  • Tartrazine Lake Yellow N
  • Tartrazine M
  • Tartrazine MCGL
  • Tartrazine N
  • Tartrazine NS
  • Tartrazine O
  • Tartrazine O Specially Pure
  • Tartrazine T
  • Tartrazine XX
  • Tartrazine XX Specially Pure
  • Tartrazine XXX
  • Tartrazine Yellow
  • Tartrazine Yellow 5
  • Tartrazine Yellow BF
  • Tartrazol BPC
  • Tartrazol Yellow
  • Tartrine Yellow O
  • Triacid Tartrazine Yellow O
  • Triacid Yellow T
  • Trinatrium-5-hydroxy-1-(4-sulfophenyl)-4-(4-sulfophenylazo)pyrazol-3-carboxylat
  • Trisodium 3-carboxy-5-hydroxy-1-p-sulfophenyl-4-p-sulfophenylazopyrazole
  • trisodium 5-hydroxy-1-(4-sulphophenyl)-4-(4-sulphophenylazo)pyrazole-3-carboxylate
  • Unitertracid Yellow TE
  • Usacert FD&C Yellow No. 5-310122
  • Usacert Yellow No. 5
  • Vitasyn Tartrazine X 90
  • Vitasyn Tartrazine XX 90
  • Vondacid Tartrazine
  • Water Yellow 1
  • Water Yellow 176552
  • Water Yellow 176571
  • Water Yellow 178758
  • Water Yellow 178819
  • Wool Yellow
  • Xylene Fast Yellow GT
  • Yellow HO 203
  • Yellow Lake 69
  • D&C Yellow 5
  • A.F. Yellow No. 4
  • Acid Leather Yellow T
  • Dye FD and C Yellow No. 5
  • EINECS 217-699-5
  • FD & C Yellow No. 5
  • FD & C Yellow No. 5 tartrazine
  • FD and C Yellow 5
  • FD&G Yellow No. 5
  • Schultz No. 737
  • 1-(4-Sulfophenyl)-4-((4-sulfophenyl)azo)-1H-pyrazole-3-carboxylic
  • Tartrazine A expo T
  • Tartrazine C Extra
  • Tartrazine Extra Pure A
  • Tartrazine FD & C Yellow #5
  • Tartrazine FD&C Yellow #5
  • Trisodium 4,5-dihydro-5-oxo-1-(4-sulfophenyl)-4-((4-sulfophenyl)azo)-1H-pyrazole-3-carboxylate
  • Trisodium salt of 3-carboxy-5-hydroxy-1-sulfophenylazopyrazole
  • Yellow No. 5
  • Yellow No. 5 FDC
  • Zlut kysela 23
  • Zlut pigment 100
  • Zlut potravinarska 4
  • UNII-I753WB2F1M
  • 117209-34-4
  • 12000-64-5
  • 1342-47-8
  • 1342-53-6
  • 134240-82-7
  • 139601-06-2
  • 154881-98-8
  • 183808-13-1
  • 191807-79-1
  • 389057-90-3
  • 469888-21-9
  • 50809-64-8
  • 642-62-6
  • 84842-94-4

Applications:

HPLC Method for Analysis of FD&C Yellow 5 (Tartrazine) on BIST A and BIST A+ Columns

March 21, 2023

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Tartrazine (FD&C Yellow 5) on BIST A and BIST A+ Columns by SIELC Technologies

HPLC Method for Analysis of  FD&C Yellow 5 (Tartrazine) on BIST A and BIST A+ Columns by SIELC Technologies
HPLC Method for Analysis of Tartrazine (FD&C Yellow 5) on BIST A and BIST A+ Columns by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Tartrazine (FD&C Yellow 5)

Tartrazine is a popular, multi-charged yellow synthetic dye used in a multitude of food and cosmetic products, including colorful sugar-coated chocolate candies. Using SIELC’s newly introduced BIST™ method, tartrazine, which dissolves and ionizes in water, can be retained on a negatively-charged, cation-exchange BIST™ A+ column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as Magnesium acetate (Mg(AcO)2), which acts as a bridge, linking the negatively charged dye to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Tartrazine can be separated, retained, and UV detected at 430 nm.

To extract the dye from the candy, place a colored sugar-coated chocolate candy in a beaker filled with about 20 mL of water. Once the dye is dissolved into the water, fill a syringe with the water and then place a filter at the end of the syringe. Depress the plunger and fill a new clean vial with the extracted dye. The Tartrazine is now ready to be analyzed.

Condition

ColumnBIST A, 4.6 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN – 75%
BufferTMEDA Formate pH 4.0 – 20 mM
Flow Rate0.5 ml/min
DetectionVis 430 nm

Description

Class of CompoundsAcid, Dye
Analyzing CompoundsTartrazine (FD&C Yellow 5)

Application Column

BIST A

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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BIST A+

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Tartrazine (FD&C Yellow 5)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of  FD&C Yellow 5 (Tartrazine) in M&M’s  on BIST A+ Column

February 16, 2023

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Tartrazine (FD&C Yellow 5) on BIST A+ by SIELC Technologies

HPLC Method for Analysis of  FD&C Yellow 5 (Tartrazine) in M&M’s  on BIST A+ Column by SIELC Technologies
HPLC Method for Analysis of Tartrazine (FD&C Yellow 5) on BIST A+ by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Tartrazine (FD&C Yellow 5)

Tartrazine is a popular, multi-charged yellow synthetic dye used in a multitude of food and cosmetic products, including colorful sugar-coated chocolate candies. Using SIELC’s newly introduced BIST™ method, tartrazine, which dissolves and ionizes in water, can be retained on a negatively-charged, cation-exchange BIST™ A+ column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as Magnesium acetate (Mg(AcO)2), which acts as a bridge, linking the negatively charged dye to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Tartrazine can be separated, retained, and UV detected at 430 nm.

To extract the dye from the candy, place a colored sugar-coated chocolate candy in a beaker filled with about 20 mL of water. Once the dye is dissolved into the water, fill a syringe with the water and then place a filter at the end of the syringe. Depress the plunger and fill a new clean vial with the extracted dye. The Tartrazine is now ready to be analyzed.

Condition

ColumnBIST A+, 3.2 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN – 70%
BufferMagnesium Acetate pH 4.0 – 20 mM
Flow Rate0.5 ml/min
DetectionVis 430 nm

Description

Class of CompoundsAcid, Dye
Analyzing CompoundsTartrazine (FD&C Yellow 5)

Application Column

BIST A+

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Tartrazine (FD&C Yellow 5)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Tartrazine on BIST A Column

December 1, 2022

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Tartrazine on BIST A Column by SIELC Technologies

HPLC Method for Analysis of Tartrazine on BIST A Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Tartrazine

Tartrazine is a popular yellow synthetic dye used in a multitude of food and cosmetic products. Using SIELC’s newly introduced BIST™ method, tartrazine, which ionizes in water, can be retained on a negatively-charged, cation-exchange BIST™ A column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged tartrazine analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. The effect of reducing the solvation layer by increasing the organic component concentration in the mobile phase can be clearly seen above, as can the effect of multi- and singly-charged buffers on retention. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, tartrazine can be retained and UV detected at 260 nm.

Condition

ColumnBIST™ A, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN
BufferTMDAP formate pH 4.0 – 5,0 mM
Flow Rate1.0 ml/min
Detection260 nm

Description

Class of CompoundsAcid, Dye
Analyzing CompoundsTartrazine

Application Column

BIST A

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
Tartrazine (FD&C Yellow 5)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Brilliant Blue FCF Tartrazine, Sunset Yellow FCF and Allura Red in Monster Energy Drinks on Newcrom BH Column

July 14, 2020

Separation type: Liquid Chromatography Mixed-mode




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Brilliant Blue FCF, also known as Blue 1, is an compound classified as a triarylmethane dye. Dry, it has the appearance of a light-blue powder, but when dissolved in water, turns deeper blue. Allura Red AC, also known as FD&C Red No. 40, is a red azo dye that is the most commonly used red dye in the United States. It is used anywhere from tattoos to children’s’ medications to drinks and food. If used in food it also has the E number E129. Allura red is sold as a dark red sodium salt, but when dissolved in water, the solution appears orange-red.Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow.The dye is banned or restricted in Norway, Finland and Sweden.
Tartrazine is a yellow food coloring agent. It’s a trisodium salt, and like many multi-charged molecules tends to exhibit significant tailing using reverse-phase HPLC columns. By using a mixed-mode column, specifically designed in mind for multiple charges, Newcrom BH can retain and separate dyes with perfect peak shape using a mobile phase of methanol (MeOH) and water with sulfuric acid (H2SO4) to facilitate ion-exchange. UV detection at 400nm.



High Performance Liquid Chromatography (HPLC) Method for Analysis of Brilliant Blue FCF, Tartrazine, Sunset Yellow FCF and Allura Red




 

Condition

Column Newcom BH, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeOH/H2O – 75/25%
Buffer H2SO4 – 0.75%
Flow Rate 1.0 ml/min
Detection UV 400 nm

Description

Class of Compounds Acid, Dyes
Analyzing Compounds Brilliant Blue FCF, Allura Red, Tartrazine, Sunset Yellow FCF

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Allura Red (Red #40)
Brilliant Blue FCF (Blue 1)
Sunset Yellow (Yellow 6)
Tartrazine (FD&C Yellow 5)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Tartrazine (Yellow 5) on Newcrom BH Column

July 1, 2020

Separation type: Liquid Chromatography Mixed-mode

 


Tartrazine is a yellow food coloring agent. It’s a trisodium salt, and like many multi-charged molecules tends to exhibit significant tailing using reverse-phase HPLC columns. By using a mixed-mode column, specifically designed in mind for multiple charges, Newcrom BH can retain tartrazine with perfect peak shape using a mobile phase of methanol (MeOH) and water with sulfuric acid (H2SO4) to facilitate ion-exchange. UV detection at 430nm.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Tartrazine

Condition

Column Newcrom BH, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeOH/H2O – 75/25%
Buffer H2SO4 – 1%
Flow Rate 1.0 ml/min
Detection UV 430  nm

Description

Class of Compounds Acid, Dyes
Analyzing Compounds Tartrazine Yellow 5

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Tartrazine (FD&C Yellow 5)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 2 Wavelengths

March 29, 2017

Sunset Yellow FCF, also known as Orange Yellow S, C.I. 15985, or Yellow 6, is a petroleum-derived orange azo dye. When in powder form, it is Reddish-orange, but when dissolved in water, the liquid becomes bright yellow. The dye is banned or restricted in Norway, Finland and Sweden. Obelisc N is a column with very polar characteristics. It contains embedded acidic and basic ionizable groups and can retain Sunset Yellow. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.

 

Condition

Column Obelisc N, 3.2×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer AmFm pH 3.0 – 70 mM
Flow Rate 0.6 ml/min
Detection UV, 430, 525 nm

 

Description

Class of Compounds
Dye, Acid, Hydrophilic, Ionizable, Zwitterionic
Analyzing Compounds Allura Red, Sunset Yellow, Tartrazine

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Analytes:
Allura Red (Red #40)
HPLC Method for Analysis of Allura Red in Children’s Mucinex Multi-Syptom Cold
HPLC Method for Analysis of Allura Red in Robitussin Cough & Cold
HPLC Method for Analysis of Allura Red in Soft Drinks 2
HPLC Method for Analysis of Allura Red in Soft Drinks 3
HPLC Method for Analysis of Allura Red in Soft Drinks 4
HPLC Method for Analysis of Allura Red in Soft Drinks 5
HPLC Method for Analysis of Allura Red, Sunset Yellow, Tartrazine in Mountain Dew Live Wire at 1 Wavelength
HPLC Method for Analysis of Brilliant Blue and Allura Red in Children’s Mucinex Night Time Multi-Symptom Cold
Sunset Yellow (Yellow 6)
Tartrazine (FD&C Yellow 5)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.